Figure 2
From: Identification of a novel scaffold for a small molecule GPR139 receptor agonist
Representative data of the 2-stage screening protocol which enabled simultaneously screening for agonist and antagonists hits by eliciting different response profiles during the initial addition of (A) buffer, (B) agonist or (C) antagonist and subsequent addition of the reference agonist compounds 1a (cmp1a). Only the second addition of 1a was measured during the screening campaign and the agonist/antagonist activity was then uncovered during subsequent detailed characterization of the hits.
