Figure 4
Epithelial-mesenchymal transition is occurred in the gingival epithelial cells during ameloblastic cytodifferentiation. (A) Expression of vimentin, a mesenchymal fibroblast marker in cells treated with cytokines for 7 days. Endogenous vimentin (α-vimentin) and actin (α-actin) were detected by western blot analysis. 1, control without treatment; 2, co-treatment with BMP4 & BMP2; 3, treatment with TGFβ-1; 4, co-treatment with BMP4, BMP2, & TGFβ-1. (B) Immunophenotyping of the gingival epithelial cells treated with cytokines. Expression of the mesenchymal cell surface was analyzed by FACS analysis after incubation with anti-CD44, anti-CD73, anti-CD90, and anti-CD146 antibodies. a, FACS histograms; b, mean fluorescence calculated from FACS data. This FACS histogram was representative of one of the three experiments using three individual cultures of gingival epithelial cells.
