Figure 1
Formation of P-bodies is induced by cell wall stress. (a) Wild-type (WT) cells transformed with plasmids expressing a GFP-tagged version of Dcp2 or Pat1 growing in YPD were exposed to 30 µg/ml Congo red (CR) or 0.8 U/ml zymolyase (ZY) for one hour (lower panel) or 1 M KCl, 3 mM H2O2 and absence of glucose for 15 min (upper panel). P-body formation was then assessed using fluorescence microscopy. (b) P-body formation was studied using the Dcp2-GFP reporter in WT, pat1∆ and edc3∆ pat1∆ cells grown as indicated above. (c) Stress granule formation was not influenced by cell wall stress. WT cells expressing either Pub1-mCherry or Pab1-GFP were treated with CR and ZY as described in a, and with 15% ethanol for 30 minutes before being observed by fluorescence microscopy. The microscopy data are presented in the left panels and the quantitation of the results in the right panels. Non-treated cells are also included in each experiment as a control (−). The histograms show the number of P-bodies per 100 cells and the percentage of cells containing P-bodies. The data reflect the average and SD values obtained from three independent experiments (n > 100 cells). Statistical significance was determined using a two-tailed, unpaired, Student’s t test by comparing with no treatment conditions (a) or with the corresponding CR or ZY data from the wild-type strain (b) (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). Scale bar, 5 μm.
