Figure 2
Time course of P-bodies formation in wild-type cells after cell wall stress. Wild-type (WT) cells expressing Dcp2-GFP as a P-body marker were treated with 30 µg/ml CR or 0.8 U/ml ZY and, in addition to the untreated control, were visualized by fluorescence microscopy at the times indicated. Scale bar, 5 μm. The histograms show the number of P-bodies per 100 cells and the percentage of cells containing P-bodies. The data reflect the average and SD values obtained from three independent experiments (n > 100 cells). Statistical significance was determined using a two-tailed, unpaired, Student’s t test by comparing with no treatment conditions for each time (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns, not significant).
