Figure 7
The induction of mRNA granules under cell wall stress conditions is associated with P-body formation. (a) Time course analysis of the appearance of MLP1-U1A mRNA granules in wild-type (WT) cells coexpressing MLP1-U1A mRNA and U1A-GFP protein after ZY exposure (0.8 U/ml) at the indicated times are shown in the graphs. The data reflect the average of mRNA granules counts from three independent experiments analysed by fluorescence microscopy. (b) Following the strategy described above, the presence of MLP1-U1A mRNA granules resulting from cell wall stress (two hours of ZY treatment) was quantified in WT and yeast strains defective in P-body formation (pat1∆ and edc3∆ pat1∆). Statistical significance was determined using a two-tailed, unpaired, Student’s t test by comparing with no treatment conditions for each time (a) or ZY data from the wild-type strain (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns, not significant).
