Figure 3
From: RNA Stabilizes Transcription-Dependent Chromatin Loops Induced By Nuclear Hormones
The CAV1 basal and E2 induced transcripts differ at their 5′ ends; the BCL2 basal and E2-induced transcripts differ at their 3′ ends. (A) CAV1 and the CAV1 mRNAs. The top panel shows the genomic structure of the CAV1 gene (red box, promoter for Start 1; green boxes, exons; blue lines, introns; yellow boxes, enhancers (EREs); blue box, polyA sites) and the E2-induced transcripts, (black lines, Start 2) and basal transcripts (red lines, Start 1). Primer for start two is shown in Supplementary Table 1 (CAV1-RNAF). This primer detects CAV1 mRNAs indicated as 202 and 203 in ref.9. The lower panel shows the basal CAV1 mRNAs in MCF7 cells and the accumulation CAV1 mRNAs after induction, using qPCR, t test: * or **p ≤ 0.001 as compared to the basal or 30 min +E2, respectively. (B) BCL2 and BCL2 mRNAs. The top panel shows the genomic structure of the BCL2 gene (red box, promoter; green boxes, exons; blue lines, introns; yellow box, enhancers (EREs); blue box, polyA sites) and the E2-induced (black lines) or basal transcripts (red lines). Primer for the long transcript originating at start 1 is shown in Supplemental Table 1 (BCL2-RNAR).The lower panel shows basal BCL2 mRNAs and accumulation of E2-dependent BCL2 mRNAs in MCF7 cells by qPCR, t test: * or **p ≤ 0.001 as compared to the basal or 30 min +E2, respectively.
