Figure 2
From: A Revised View on Growth and Remodeling in the Retinal Vasculature

Development of the mouse retinal vasculature from postnatal day p3 to p120. (A) Retinal flat mounts of p3, p7, p10, p30, p60 and p120 old mice with close up. Scale bar overview: 1 mm; close up: 100 µm. (B) Binarized images of Cldn5-eGFP+ retinal flatmounts from p3 to adulthood (p120) (left sides) and corresponding heatmaps of the vascular area fraction (right sides). Scale bar 1 mm. (C) Histograms of retinal heatmaps. (D) Quantitative evaluation of vascular outgrowth, area fraction, segment length, number of branches, nearest neighbor distance (NND) and variability of NND from p3 to adulthood. The animal number is n = 4 (p3, p7, p10) and n = 3 (p30, p60, p120).