Figure 1
(a) Mice with monoallelic Nampt deletion from rod photoreceptors (Nampt−rod/WT; N = 7) were similarly susceptible to streptozotocin (STZ)-induced hyperglycemia compared to NamptF/WT controls (N = 4; 2-tailed, unpaired t-test). (b–d) At 11 weeks after STZ induction, Nampt−rod/WT mice (N = 6–7) did not exhibit any signs of retinal dysfunction compared to NamptF/WT controls (N = 4) based on their scotopic a-wave, scotopic b-wave, or photopic b-wave amplitudes (2-way mixed ANOVA). (e,f) At 6 weeks after STZ induction in wild-type (WT) mice, there were no changes in retinal SIRT3 or SIRT5 protein levels as measured by immunoblotting shown in (e) with quantifications shown in (f). Open circles depict individual mice (a); graphs depict mean ± S.E.M. (b–d) or mean + S.E.M. (f) Each lane represents an individual mouse; the entire gel is depicted without splicing or cropping (e).
