Figure 8
(a) SIRT3/SIRT5 double knockout mice (3KO5KO) were equally susceptible to streptozotocin (STZ)-induced hyperglycemia compared to littermate controls of various genotypes. (b–d) At 6 weeks after STZ induction, hyperglycemic 3KO5KO mice did not exhibit any retinal dysfunction based on their scotopic a-wave, scotopic b-wave, and photopic b-wave amplitudes compared to hyperglycemic littermate controls (N = 4–7/group; 2-way mixed ANOVA). (e) Representative histological images show that hyperglycemic 3KO5KO mice did not exhibit any changes in retinal morphology compared to hyperglycemic littermate controls. (f–h) Hyperglycemic 3KO5KO mice did not have any significant changes in scotopic a-wave, scotopic b-wave, or photopic b-wave implicit times compared to hyperglycemic littermate controls (N = 4–7/group; 2-way mixed ANOVA). (i,j) Hyperglycemic 3KO5KO mice had significant reductions in scotopic but not photopic oscillatory potential (OP) amplitudes compared to hyperglycemic littermate controls (N = 4–7/group; 2-way mixed ANOVA with Bonferroni post-hoc test). Open circles depict individual mice (a); graphs depict mean ± S.E.M. (b–d, f–j) (RMS: root mean square; **P < 0.01; ***P < 0.001; red asterisks indicate significant differences compared to Sirt3+/−Sirt5+/− mice after post-hoc testing).
