Figure 1
From: Unexpected genomic rearrangements at targeted loci associated with CRISPR/Cas9-mediated knock-in
Comparison of cHR and Cas9n knock-in performance in mouse embryonic stem cells. (A) Number of experiments and loci targeted in mouse ES cells using Cas9n technology (Cas9n) and cHR (see Sup Table for further details). (B) Box plots of percentages of positive clones detected by 5′PCR (left) or 3′PCR (right) for all experiments at all loci using Cas9n or cHR. (C) Box plots of percentages of clones validated by Southern Blot (SB; left) or sequencing (right) of the targeted locus for all experiments at all loci using Cas9n or cHR. (D) Box pots of Discovery Index (DI, left) and Validation Index (VI, right) for all experiments at all loci using Cas9n or cHR. DI and VI represents the technology’s efficiency to produce PCR-positive clones and fully validated clones, respectively.
