Figure 2
From: Unexpected genomic rearrangements at targeted loci associated with CRISPR/Cas9-mediated knock-in
Knock-In efficiency for inserts of different size with Cas9n and cHR. (A) Number of experiments and loci targeted in mouse ES cells using Cas9n technology or cHR for inserts of various size (see Sup Table for further details). (B) Box plots of Discovery Index (DI, top) and Validation Index (VI, bottom) for small (<2 kb) and bigger inserts (>2 kb). (C) Box plots of Discovery Index (DI, top) and Validation Index (VI, bottom) for large (>8 kb) and smaller inserts (<8 kb). Note that Cas9n’s DI is superior to cHR’s for all insert sizes whereas cHR’s VI is higher than Cas9n’s in all conditions except for inserts >8 kb.
