Figure 3
From: Unexpected genomic rearrangements at targeted loci associated with CRISPR/Cas9-mediated knock-in
Homology arm length and Cas9n enzyme concentration effect on Cas9n efficiency. (A) Number of experiments and loci targeted in mouse ES cells using Cas9n technology with short (sHA) or long homology arms (lHA), low (LC) and high concentration (HC) of enzyme, and cHR (see Sup Table for further details). (B) Box plots of Discovery Index (DI, top) and Validation Index (VI, bottom) for sHA and lHA. (C) Box plots of Discovery Index (DI, left) and Validation Index (VI, right) for LC and HC. Note that HA length does not affect Cas9n’s efficiency. HC shows higher DI than LC but the VI is still inferior to cHR’s. R: μg Cas9n/million cells.
