Figure 5
From: Insights into the complex role of GRAS transcription factors in the arbuscular mycorrhiza symbiosis
Histochemical localization of the promoter activities of selected MtGras genes. The promoter activities of MtGras1 (A: a–s), MtGras4 (A: e–h), MtGras6 (A: i–l), MtGras7 (A: m–p), and MtRad1 (A: q–t) were recorded in transgenic, mycorrhized roots of M. truncatula A17 wild type (A: a,b,e,f,i,j,m,n,q, and r) and pt4-2 roots (c,d,g,h,k,l,o,p,s, and t). The promoter activities of MtGras1 (B: a–d) and MtGras7 (B: e–h) were furthermore recorded in transgenic, mycorrhized M. truncatula pt4-2 roots, showing a premature arbuscule degeneration (PAD; B: a,b,e,f) or Non-PAD phenotype (B: c,d,g,h). GUS stainings were performed for 4–8 hours. Alexa WGA Fluor 488 stainings are shown to visualize fungal colonization. Close-up views of PAD and Non-PAD arbuscules are shown inside red angles.
