Table 2 Steady state distribution of folate (in percentage of total folate) for different scenarios with respect to MTHFS and SHMT activity.
THF | 10fTHF | CHF | CH2F | DHF | 5mTHF free | 5mTHF bound | 5mTHF total | 5fTHF free | 5fTHF bound | 5fTHF total | |
|---|---|---|---|---|---|---|---|---|---|---|---|
Standard MTHFS and regular SHMT activity (Condition “A”) | 0.27 | 37.65 | 7.49 | 2.12 | 0.04 | 27.77 | 19.39 | 47.16 | 2.20 | 3.07 | 5.27 |
0.5× MTHFS and regular SHMT activity (Condition “B”) | 0.25 | 37.17 | 7.40 | 2.10 | 0.04 | 26.46 | 17.29 | 43.75 | 4.03 | 5.27 | 9.29 |
0× MTHFS and regular SHMT activity (Condition “C”) | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 76.38 | 23.62 | 100.00 |
0× MTHFS and SHMT:CHF → 5fTHF activity turned off (Condition “D”) | 0.33 | 39.30 | 7.81 | 2.19 | 0.04 | 27.05 | 23.28 | 50.33 | 0.00 | 0.00 | 0.00 |
Standard MTHFS and regular SHMT activity without MTHFS inhibition by 10fTHF (Condition “E”) | 0.30 | 38.22 | 7.60 | 2.14 | 0.04 | 29.36 | 22.32 | 51.68 | 0.01 | 0.01 | 0.02 |
