Figure 1 | Scientific Reports

Figure 1

From: p300/CBP-associated factor promotes autophagic degradation of δ-catenin through acetylation and decreases prostate cancer tumorigenicity

Figure 1

PCAF and HDACs regulate the acetylation status and levels of δ-catenin. (A,B) PCAF decreases δ-catenin levels. HEK293T (A) and δ-catenin-overexpressing CWR22Rv-1 (Rv/δ) (B) cells were transfected as indicated, and cell lysates were subjected to immunoblotting. (C–F) PCAF interacts with and acetylates δ-catenin. HEK293T cells were transfected as indicated, and cell lysates were subjected to immunoprecipitation with anti-δ-catenin (C), anti-Flag (D), or anti-acetylated-lysine (E,F), followed by immunoblotting of precipitated proteins. (G–I) HDACs deacetylate and upregulate δ-catenin. HEK293T cells were transfected as indicated, and cell lysates were subjected to immunoblotting (G) or immunoprecipitation with anti-acetylated-lysine (H). At 12 h post-transfection of HEK293T cells with GFP-δ-catenin and HDAC1, cells were treated with 0.2 µM trichostatin A (TSA) or transfected with Flag-PCAF, and incubated for 24 h, followed by immunoblotting with anti-δ-catenin and anti-Flag antibodies (I). α-Tubulin or ß-actin was used as a loading control. Relative δ-catenin/actin/HDACs ratios from three different experimental results are shown as a bar graph (G). Values are presented as the mean ± SEM. “−”, Mock transfection or vehicle treatment.

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