Figure 4 | Scientific Reports

Figure 4

From: p300/CBP-associated factor promotes autophagic degradation of δ-catenin through acetylation and decreases prostate cancer tumorigenicity

Figure 4

Multiple domains of δ-catenin are responsible for PCAF-mediated δ-catenin downregulation. (A) Schematic diagram of different constructs of full length GFP-δ-catenin and its deletion mutants (ΔC207, ΔN85–325, ΔN&ΔC, 1–690, 691–1040, 1–640, 1–600, 1–579, and 1–499) and the distribution of Lys residues on these deletion mutants. Lys residues (Lys1049, Lys1050, and Lys1158) form the ubiquitin-dependent degron of δ-catenin. (B) Both 1–690 and 691–1040 deletion mutants of δ-catenin were downregulated by PCAF. HEK293T cells were transfected with different combinations of the indicated plasmids, and cell lysates were subjected to immunoblotting. Actin was used as a loading control. Relative values of δ-catenin/actin ratios from at least three independent experiments are shown as a bar graph in each panel (ii). Values are presented as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; NS, no significant difference compared with the control group. “−”, Mock transfection.

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