Table 1 Characteristics of sources of samples positive for blaCTX-M, blaOXA-48-type, and putative blaSHV or blaTEM genes; aGene variant or cluster with greatest homology in GenBank; bCPWS: Community public water system, NCPWS: Non-community public water system; cSamples collected from the same water systems: A1 and A2; D1, D2, and D3; E2, E5, and E6; d100% nucleotide homology to uncultured bacterium clones; eGene sequence is similarly homologous with both ESBL and non-ESBL blaTEM genes, but exhibits an ESBL phenotype fBlended water is a composite of ground water and treated surface water.
Sample ID | Gene variant or group with highest DNA sequence similaritya | Gene homology with reference sequence | Protein homology with reference sequence | GenBank Nucleotide Reference (GenBank Protein reference) | Organism | E. coli present/absent in sample | System typeb | Water source | GenBank Accession Number |
|---|---|---|---|---|---|---|---|---|---|
A1c | CTX-M-205 | 79% | 86% | MG028655 (ATJ25945.1) | Citrobacter freundii- complex | Absent | CPWS | Ground | MG560059 |
A2c | CTX-M-205 | 80% | 88% | MG028655 | Citrobacter freundii- complex | Present | CPWS | Ground | MG560060 |
A3a | CTX-M-40 or CTX-M-63 | 84% | 94% | NG_048991 NG_049014 | Kluyvera georgiana | Present | Private | Ground | MG560061 |
A3b | OXA-181 | 100% | 100% | CP023897 | Acinetobacter baumannii complex | Present | Private | Ground | MG560077 |
A4 | CTX-M-40 or CTX-M-63 | 98% | 99% | NG_048991 NG_049014 | Kluyvera georgiana | Absent | CPWS | Ground | MG560062 |
A5 | CTX-M-40 or CTX-M-63 | 84% | 94% | NG_048991 NG_049014 | Kluyvera georgiana | Absent | CPWS | Ground | MG560063 |
A6 | CTX-M-9 family | 86%d | 95% | NG_049028.1 | Not Isolated | Absent | CPWS | Surface | MG560055 |
B1 | CTX-M-10 or CTX-M-34 | 100% | 100% | NG_048898 NG_048984 | Kluyvera species | Present | Private | Ground | MG560064 |
B2 | CTX-M-40 or CTX-M-63 | 96% | 95% | NG_048991 NG_049014 | Not Isolated | Present | Private | Unknown | MG560056 |
B3a | CTX-M-12 | 100% | 100% | DQ821704 | Not Isolated | Present | Private | Ground | MG560057 |
B3b | OXA-48b | 100% | 100% | KU820807 | Not Isolated | Present | Private | Ground | MG560068 |
B4 | OXA-48b | 100% | 100% | KU820807 | E. coli | Present | Private | Ground | MG560078 |
B5 | OXA-181 | 85%d | 93% | KJ620504 | E. coli | Present | Private | Ground | MG560079 |
B6 | OXA-48b | 100% | 100% | KU820804 | Shewanella species | Present | Private | Ground | MG560080 |
B7 | CTX-M-3 | 100% | 100% | KU200455 | Klebsiella oxytoca | Present | Private | Ground | MG560065 |
B8 | OXA-181 | 85% | 94% | KJ620504 | Not Isolated | Present | Private | Ground | MG56006 |
B9 | OXA-48b | 100% | 100% | KU820804 | Shewanella species | Present | Private | Ground | MG560081 |
B10 | OXA-252 | 100% | 100% | CP022089 | Not Isolated | Present | Private | Ground | MG560070 |
B11a | OXA-48b | 99% | 99% | KC902850 | Shewanella putrefaciens; | Absent | NCPWS | Ground | MG560082 |
B11b | OXA-48b | 99% | 99% | KC902850 | Pandoraea sputorum | Absent | NCPWS | Ground | MG560083 |
B12 | TEM-1 variante | 99% | 99% | KU664682.1 | E. coli | Present | Private | Ground | MG560088 |
B13 | OXA-48b or OXA-252 | 100% | 100% | KU820804 NG_050608.1 | Shewanella species | Present | Private | Ground | MG560084 |
C1 | CTX-M-3 | 99% | 99% | Y10278 | Not Isolated | Absent | CPWS | Surface | MG560058 |
C2 | CTX-M-2-like | 89% | 96% | KX377894 | Kluyvera ascorbata | Absent | CPWS | Ground | MG560066 |
D1c | OXA-48b | 100% | 100% | KU820807 | Providencia rettgeri | Absent | NCPWS | Ground | MG560085 |
D2c | OXA-252 | 100% | 100% | NG_050608 | Not Isolated | Present | NCPWS | Ground | MG560071 |
D3c | OXA-199 | 99% | 99% | NG_049495 | Not Isolated | Present | NCPWS | Ground | MG560072 |
E1a | CTX-M-36 | 99% | 100% | NG_048986 | Klebsiella oxytoca | Absent | CPWS | Blendf | MG560067 |
E1b | OXA-48b | 100% | 100% | KU820807 | Not Isolated | Absent | CPWS | Blendf | MG560073 |
E2c | OXA-48b | 100% | 100% | KU820807 | Not Isolated | Absent | CPWS | Blendf | MG560074 |
E3 | OXA-252 | 100% | 100% | NG_050608 | Pseudomonas species | Absent | CPWS | Blendf | MG560075 |
E4 | OXA-48b | 100% | 100% | KU820802 | Not Isolated | Absent | CPWS | Blendf | MG560076 |
E5c | OXA-48b | 100% | 100% | KU820807 | Pseudomonas koreensis | Absent | CPWS | Blendf | MG560086 |
E6c | OXA-252 | 100% | 100% | KU820800 | Pseudomonas putida | Absent | CPWS | Blendf | MG560087 |
F1 | SHV-38 | 100% | 100% | NG_050077 | Klebsiella pneumoniae | Present | NCPWS | Ground | MG560089 |
