Figure 1
From: ApoE-fragment/Aβ heteromers in the brain of patients with Alzheimer’s disease
Quantification and characterization of the Aβ forms found in the cortex of AD patients. (A) Representative western blot of cortex proteins extracted from control and AD patients (each pit representing a different patient) and revealed with an anti-Aβ full length (PA3-16761, green). GAPDH (red) was revealed as an internal control for each deposit. (B) Bar graph representing the statistical analysis of Aβ/GAPDH ratio measured with PA3 antibody for 18 and 16 kDa forms in control versus AD brain samples. (C) Bar graphs representing the statistical analysis of the Aβ/GAPDH ratio measured with 6E10 antibody for the 18, 16 and 12 kDa forms in control versus AD brain samples. The number of samples analyzed is indicated in parentheses, significant statistical differences are marked by *(p < 0.05) and **(p < 0.005) as calculated by Mann-Whitney test. (D) Western blots of cortex from a control (Braak 1, Thal 0) and an AD patient (Braak 6, Thal 4) revealed with 3 different anti-Aβ antibodies (G2-11, MOAB2, 6E10). All fours antibodies recognized the 18 and 16 kDa forms of Aβ as compiled in (E) while the PA3 antibody poorly recognize the 12 kDa Aβ form. The lanes illustrated in Fig. 1D have been cropped from Supplementary Fig. S3A.
