Figure 2

Effects of pre- and post-anthesis NO3− availability on end of cycle characteristics. Plants were grown in hydroponics. Ctrl: control plants grown on 1 mM NO3− during the entire life cycle. Pre-anthesis: plant grown on 0.1 or 10 mM NO3− (LN and HN, respectively) until anthesis, and subsequently on 1 mM NO3−. Post-anthesis: plant grown on 1 mM NO3− until anthesis, and subsequently on LN or HN. (a) Grain yield per plant. (b) Shoot Dry Weight (DW). (c) Grain number per plant. (d) DW of individual grain. (e) Spike number per plant. (f) Grain number per spike. The boxplots represent minimum, 1st quartile, median, 3rd quartile and maximum values. Letters indicate statistical groups (Non-parametric ANOVA, p < 0.05). Number of biological replicates for each condition are indicated above the charts.