Figure 6 | Scientific Reports

Figure 6

From: Developmental and physiological responses of Brachypodium distachyon to fluctuating nitrogen availability

Figure 6

Characterization of High and Low NO3 Transport Systems (HATS and LATS, respectively). Plants were grown hydroponically on 2 mM NO3 for 7 days, then N starved for 8 days (−N, open circles and dashed lines) or N starved for 6 days and induced with 1 mM NO3 for 24 h (+NO3, closed circles and solid lines). (a) 15NO3 influx at external concentrations ranging from 0.01 to 10 mM 15NO3. (b) 15NO3 influx at external concentrations ranging from 0.01 to 0.2 mM 15NO3. HATS kinetics were fitted with Michaelis-Menten curves (Influx = Vmax * [15NO3]/(Km + [15NO3]). Vmax and Km values were obtained by linear regression on the Lineweaver-Burk representation (1/Influx = a * (1/[15NO3]) + b, with Vmax = 1/b and Km = a/b). cHATS and iHATS: constitutive and inducible HATS (respectively). (c) iHATS influx at external concentrations of 0.01–0.2 mM 15NO3, obtained by subtracting −N values from +NO3 values in panel b and fitted with Michaelis-Menten curve. (d) LATS 15NO3 influx at external concentrations ranging from 0.5 to 10 mM 15NO3, obtained by subtracting HATS Vmax values (panel b) from corresponding influx values (panel a). LATS kinetics were fitted with linear curves. cLATS and iLATS: constitutive and inducible LATS (respectively). (e) iLATS curve, obtained by subtracting values from −N condition to values from +NO3 condition in panel d, fitted with a linear curve. Values correspond to the mean of 5 biological replicates +/−SD.

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