Figure 3
From: Characterization of polydactyly chondrocytes and their use in cartilage engineering
Characterization of infant polydactyly tissue and cells. (a) Histological analysis of infant polydactyly cartilage tissue. The images are oriented to present the cartilage surface on the top. Scale bar: 100 µm. (b) Growth kinetics of infant and adult chondrocytes expressed as population doubling levels (PDLs) over time. (c) Growth rate averages for infant chondrocytes compared to adult chondrocytes, calculated as population doublings per day, **p < 0.01. (d) Morphology of infant and adult chondrocytes after 5 and 7 days of 2D culture. Scale bar: 100 µm. (e–g) Collagen 2 (COL2), collagen 1 (COL1) and chondrododulin-1(CHM1) expression after sequential passaging. Gene expression was normalized against the reference gene RPL13a with one infant chondrocyte donor at passage 0 chosen as the calibrator sample.
