Figure 3 | Scientific Reports

Figure 3

From: The deubiquitinating enzyme USP25 binds tankyrase and regulates trafficking of the facilitative glucose transporter GLUT4 in adipocytes

Figure 3

siRNA mediated depletion of USP25 in adipocytes results in reduced levels of GLUT4 protein. (A) 3T3-L1 adipocytes were treated with 50 nM siRNA against either TNKS or USP25, or a scrambled control siRNA (Scr) using Mirus TransIT TKO reagent on days 6 and 7 post-differentiation before being harvested on day 8 and subject to immunoblot analysis for the indicated proteins. (B) ImageJ software was used to quantify levels of GLUT4 and IRAP, normalised against GAPDH, in cells treated with siRNA against TNKS or USP25, expressed as a percentage of their levels in cells treated with the scrambled control siRNA. Error bars represent ± SEM, n = 3; asterisk denotes significance. Full-length blots/gels are presented in the Supplementary Information.

Back to article page