Table 1 Knee joint damage, percent body fat, synovial fluid leptin, endotoxin, composite insulin sensitivity index, serum lipid profiles, and cecal microbiota for rats treated in the chow, HFS, HFS + F, HFS + E, HFS + F + E groups at the end of the experimental protocol.

From: Protective effect of prebiotic and exercise intervention on knee health in a rat model of diet-induced obesity

	Chow	HFS	HFS + F	HFS + E	HFS + F + E	Two way Anova							One way Anova
						F		E		F * E		Adjusted R²	p-value	partial η²	Observed Power
						p-value	partial η²	p-value	partial η²	p-value	partial η²	Adjusted R²	p-value	partial η²	Observed Power
Knee joint integrity	19.1 ± 4.7	47.9 ± 3.2	20.1 ± 3.5	13.6 ± 2.4	12.3 ± 2.4	<0.001	0.361	<0.001	0.539	<0.001	0.318	0.666	<0.001	0.637	1.000
Percent body fat, %	19.0 ± 1.1	29.9 ± 1.9	26.9 ± 1.6	26.1 ± 1.6	21.6 ± 2.0	0.045	0.088	0.016	0.125	0.690	0.004	0.141	0.001	0.299	0.962
SF: leptin, pg/mL	63.8 ± 8.5	251.8 ± 48.7	130.5 ± 20.7	125.4 ± 24.1	113.1 ± 18.3	0.045	0.092	0.019	0.125	0.098	0.064	0.188	0.001	0.304	0.959
Endotoxin, EU/ML	4.3 ± 0.6	8.2 ± 0.9	3.7 ± 0.6	3.4 ± 0.5	4.2 ± 0.6	0.006	0.161	0.002	0.200	<0.001	0.272	0.441	<0.001	0.432	0.999
CISI	0.60 ± 0.08	0.25 ± 0.04	0.52 ± 0.04	0.54 ± 0.07	0.56 ± 0.06	0.011	0.153	0.003	0.203	0.016	0.14	0.315	0.001	0.343	0.976
Total Cholesterol (mmol/L)	1.40 ± 0.10	1.94 ± 0.10	1.69 ± 0.11	1.85 ± 0.08	1.56 ± 0.08	0.005	0.179	0.244	0.033	0.792	0.002	0.156	0.001	0.36	0.956
HDL Cholesterol (mmol/L)	1.12 ± 0.16	1.67 ± 0.09	1.70 ± 0.11	1.64 ± 0.05	1.46 ± 0.06	0.359	0.019	0.108	0.058	0.208	0.036	0.044	0.001	0.297	0.96
LDL Cholesterol (mmol/L)	0.03 ± 0.02	0.04 ± 0.02	0.02 ± 0.02	0.03 ± 0.01	0.01 ± 0.01	0.230	0.033	0.567	0.008	0.930	<0.001	0.026	0.768	0.035	0.148
Non-HDL Cholesterol (mmol/L)	0.24 ± 0.03	0.34 ± 0.05	0.22 ± 0.05	0.24 ± 0.04	0.18 ± 0.03	0.031	0.160	0.091	0.102	0.512	0.016	0.183	0.052	0.248	0.67
Triglycerides (mmol/L)	0.64 ± 0.09	1.58 ± 0.16	0.79 ± 0.11	0.81 ± 0.07	0.71 ± 0.07	<0.001	0.310	<0.001	0.291	0.002	0.207	0.501	<0.001	0.543	1.000
Ratio: Total Chol/HDL	1.10 ± 0.03	1.17 ± 0.04	1.05 ± 0.01	1.13 ± 0.02	1.06 ± 0.02	0.003	0.195	0.545	0.009	0.345	0.022	0.158	0.031	0.198	0.741
Ratio: Tryg/HDL	0.50 ± 0.06	1.03 ± 0.21	0.48 ± 0.03	0.50 ± 0.04	0.49 ± 0.05	0.022	0.122	0.032	0.107	0.025	0.117	0.240	0.002	0.296	0.941
Cecal Microbiota
Bacteroides/Prevotella	11.91 ± 1.48	4.26 ± 0.39	13.75 ± 2.46	5.95 ± 0.49	12.78 ± 2.26	<0.001	0.344	0.835	0.001	0.437	0.014	0.306	<0.001	0.346	0.988
Bifidobacterium	0.08 ± 0.02	0.26 ± 0.05	11.01 ± 1.91	0.21 ± 0.05	10.87 ± 1.40	<0.001	0.649	0.934	<0.001	0.973	<0.001	0.625	<0.001	0.680	1.000
Enterobacteriaceae	0.16 ± 0.03	0.85 ± 0.19	0.82 ± 0.13	0.52 ± 0.10	0.34 ± 0.08	0.412	0.015	0.003	0.178	0.551	0.008	0.139	0.001	0.292	0.956
Lactobacillus	3.65 ± 0.63	0.36 ± 0.11	3.07 ± 0.56	0.26 ± 0.07	2.37 ± 0.32	<0.001	0.552	0.224	0.033	0.368	0.018	0.532	<0.001	0.569	1.000
C. coccoides (cluster XIV)	7.20 ± 0.43	7.99 ± 0.63	8.07 ± 1.12	9.97 ± 0.67	8.03 ± 1.02	0.301	0.024	0.279	0.027	0.260	0.029	0.013	0.256	0.097	0.397
C. leptum (cluster IV)	14.08 ± 0.97	15.36 ± 1.70	3.49 ± 1.04	12.58 ± 1.14	4.27 ± 0.72	<0.001	0.615	0.410	0.015	0.147	0.047	0.598	<0.001	0.640	1.000
C. cluster I	1.23 ± 0.18	1.19 ± 0.24	0.50 ± 0.04	0.86 ± 0.07	0.44 ± 0.04	<0.001	0.296	0.141	0.049	0.309	0.024	0.285	<0.001	0.360	0.992
C. cluster XI	0.11 ± 0.01	0.30 ± 0.07	0.18 ± 0.05	0.28 ± 0.07	0.08 ± 0.02	0.006	0.157	0.264	0.028	0.499	0.010	0.128	0.017	0.208	0.810
Roseburia	0.66 ± 0.32	0.24 ± 0.08	1.98 ± 0.24	0.14 ± 0.05	1.91 ± 0.32	<0.001	0.624	0.679	0.004	0.936	<0.001	0.599	<0.001	0.577	1.000
Methanobrevibacter	0.01 ± 0.00	0.01 ± 0.00	<0.001	0.01 ± 0.00	0.00 ± 0.00	<0.001	0.592	0.980	<0.001	0.111	0.057	0.575	<0.001	0.594	1.000
Akkemansia muciniphila	1.08 ± 0.50	1.25 ± 0.26	0.21 ± 0.11	1.21 ± 0.25	0.14 ± 0.06	<0.001	0.407	0.786	0.002	0.934	<0.001	0.367	0.001	0.300	0.963
Faecalibacterium prausnitzii	0.31 ± 0.05	0.95 ± 0.23	0.21 ± 0.08	0.52 ± 0.11	0.23 ± 0.05	<0.001	0.248	0.139	0.049	0.107	0.058	0.26	0.001	0.314	0.973
Collinsella aerofaciens	0.02 ± 0.00	0.02 ± 0.00	0.04 ± 0.0.01	0.02 ± 0.00	0.01 ± 0.01	0.301	0.024	0.200	0.037	0.118	0.055	0.047	0.185	0.112	0.462

Values are means ± SEM. Cecal microbiota data are shown as means ± SEM expressed as the relative abundance (%) of bacterial taxa per total bacteria (16S rRNA gene copies of microbial group/total bacteria 16S rRNA gene copies). Chow: chow control diet; HFS: high fat/high sucrose diet; F: prebiotic fibre; E: aerobic exercise. HDL: high density lipoprotein; LDL: low density lipoprotein; Chol: cholesterol. Trig: triglycerides.

Back to article page

Table 1 Knee joint damage, percent body fat, synovial fluid leptin, endotoxin, composite insulin sensitivity index, serum lipid profiles, and cecal microbiota for rats treated in the chow, HFS, HFS + F, HFS + E, HFS + F + E groups at the end of the experimental protocol.

Search

Quick links