Figure 1
From: Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions
Bimolecular fluorescence complementation in chemotaxis. (A) Simplified scheme showing the major interacting partners in the E. coli chemotaxis signaling and the reconstitution of eGFP from interacting split versions of CheZ-NeGFP and CheY~P-CeGFP. Note how exposure to attractant (SER, serine) is expected to temporally reduce CheY~P pools, whereas exposure to repellent (Ni2+) is increasing those. (B) Visible formation of both polar as well as mid-cell eGFP foci (arrows) in E. coli strain 4703 expressing CheZ-NeGFP and CheY~P-CeGFP from the PJJ promoter grown in absence of any chemo-attractant or -repellent. (C) Average number of eGFP foci numbers in a variety of E. coli strains differing in promoter driving CheZ-NeGFP and CheY~P-CeGFP expression, as well as carrying instability tags on eGFP. (D) Superposition of eGFP foci positions and their relative intensity across n = 100 cells of E. coli strain 4703.
