Figure 3
From: Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions
Colocalization of reconstituted CheY-CheZ-eGFP foci with FliM-mCherry. (A) Representative images of E. coli 5921 expressing both FliM-mCherry and cheY-Cegfp-cheZ-Negfp from PJJ (on pCRO9, top series), and after foci detection (local background subtraction by SuperSegger analysis, lower series). (B) Superposition of FliM-mCherry and Split-eGFP foci positions among 180 cells, plotted along a normalized E. coli cell. (C) Distribution of number of FliM-mCherry and split-eGFP foci per cell and the proportion of overlapping foci at less than 2 or 3 pixel resolution (120 or 180 nm).
