Table 2 Primer list.
From: Probing chemotaxis activity in Escherichia coli using fluorescent protein fusions
Primer | Target | Sequence (5′–3′)a |
|---|---|---|
130719 | cheY | CCGGACAGGGGATCCGTATTTAAATCAGGAGTGTGAAATGGC |
130720 | cheY | TAGTCGCATGACGTCCCCATGCCCAGTTTCTCAAAG |
130721 | cheZ | GACTTCACTAGTGAGGATGCGACTATGATGCAACCATC |
130722 | cheZ | CTTTACTCCTCGAGCCAGAGCCAGAGCCACCAAATCCAAGACTATCCAACAAATCG |
130723 | 5′-end of egfp | ACTGTAAGCTTATTGTTTGTCTGCCATGATGTATACATTG |
130724 | 3′-end of egfp | CTGGGCATGGGGACGTCGGGTGGAAGCGGTAAGAATGGAATCAAAGTTAACTTC |
130725 | 3′-end of egfp | GGTACGGGAAGCTTATCGCACTAGTTATTTGTATAGTTCATCCATGCCATGT |
130726 | 5′-end of egfp | GGCTCTGGCTCGAGGAGTAAAGGAGAAGAACTTTTCACTGG |
170915 | fliM | TTTCACTGACCGGATCCAAATCAGGAGTGTGAAATGGGCGATAGTATTCTTTCTCAAGC |
170916 | fliM | TTTCGACCGGTGACGCGTAACGTTCGAATTTTCCGGAAGTTTGGGCTGTTCCTCGTTCAGAGAAT |
