Figure 4
Subcellular localization of mTRQ2-MerC-SYP121 in the roots of the pEpi plants. (a) FM4-64 stained plasma membranes and mTRQ2 in the elongation zones of the roots of the pEpi lines 8 and 20. FM4-64 was applied for 3–5 min before laser scanning microscopic analyses. The areas indicated with white dashed lines are subjected to the signal profiling analyses in (b). White arrows mark the outer plasma membrane regions (PMout) which correspond to the FM4–64 peaks in (b) indicated with red dashed lines. Scale bars = 10 µm. Epi, epidermis; Cor, cortex; End, endodermis; Ste, stele. (b) Quantification of signal profiles of FM4-64 and mTRQ2 across the epidermal cell regions indicated with white dashed lines in (a). Epi, epidermis; Cor, cortex; PMout, outer plasma membrane regions.
