Table 1 Summary of previous studies showing the effectiveness of hydrogen inhalation using neonatal and adult animal models (P = post-natal day).
Animal model | Method of administration | Outcomes | |
|---|---|---|---|
Nemeth et al. (2016) | <1-day-old piglets | Inhalation of 2.1% H2 for 4 h (24-h survival) | Enhanced recovery of EEG, significant preservation of neurons, and reduction of oxidative markers |
Hayashida et al. (2014) | Adult rats | Inhalation of 1.3% H2 for 2 h (7-day survival) | Rescued neuronal death and suppressed microglia activation in the hippocampus and cerebral cortex Improved animal survival and neurological recovery in post-cardiac arrest rats |
Oláh et al. (2013) | 1–2-day-old piglets | Inhalation of 2.1% H2 for 4 h (24-h survival) | Recovery of EEG function, modest neuroprotection in histopathology, and alleviated delayed neurovascular dysfunction |
Matchett et al. (2009) | P10 rats | Inhalation of 2.9% H2 for 4 h (24-h survival) | Did not ameliorate moderate-to-severe ischaemic damage |
Cai et al. (2008) | P7 rats | Inhalation of 2% H2 for 30, 60 or 120 min (24-h survival) | Provided brain protection in mild insult via inhibition of neuronal apoptosis in a duration-dependent manner |
Ohsawa et al. (2007) | Adult rats | Inhalation of 1%, 2% or 4% H2 for 120 min (12-h, 3-day, and 7-day survival) | Oxidative markers substantially reduced in H2-treated rats and a distinct H2-dependent decrease in the accumulation of microglia |
