Figure 7 | Scientific Reports

Figure 7

From: The contraceptive medroxyprogesterone acetate, unlike norethisterone, directly increases R5 HIV-1 infection in human cervical explant tissue at physiologically relevant concentrations

Figure 7

MPA significantly increases relative frequency of CD4+ cells and CD14+ CD69+ expressing cells after 7 days. Ectocervical explant tissue were stimulated in parallel with vehicle (EtOH) or 100 nM MPA or 100 nM NET for 7 days after which the relative levels of (a) CD3+, CD4+ and CD8+ and CD14 T cells (b) CD69 and (c) CCR5 or (d) CD69, CCR5 expressing CD3+, CD4+ and CD8+ and CD14 T cells were determined using flow cytometry. Results show the fold changes of frequency of 8 independent experiments, where the % cells for vehicle for each donor sample was set to 1 and the relative frequency for matched MPA and NET incubations was calculated relative to that donor vehicle value. (e) CD4/CD8 ratios of the 8 independent experiments were determined. Histograms are representative of mean ± SEM. Statistical significance was determined by using a non-parametric Kruskal-Wallis one-way ANOVA with a Dunn’s post-test with * or ** denoting p < 0.05 or p < 0.01, respectively.

Back to article page