Figure 3

rTF elicits a protective immune response in mice. BALB/c mice were immunized with rTF emulsified with CFA and subsequently boosted with rTF emulsified in IFA. Control mice were immunized with the adjuvant only. Two weeks following the final immunization, mice were challenged intranasally with a lethal dose (1 × 108 CFU) of WU2 and survival was monitored daily. The figure is a summary of three independent experiments. The extent of survival was determined by using the Log-rank (Mantel-Cox) test (n = 45 in each group; ***p = 0.0005).