Figure 3 | Scientific Reports

Figure 3

From: The Parkinson’s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4

Figure 3

Induced phosphorylation of Akt in Lrrk2 deficient cells. (a) Western blot analysis of P-Akt Thr 308 and Ser473 in protein extracts from monocytes of 1 year old Lrrk2 deficient and wild-type mice stimulated with insulin at different time points (0 to 40 min) after stimulation. (b) Quantification of P-Akt Thr308 (b, top) and P-Akt Ser473 (b, button) intensity in protein extracts from monocytes of 1 year old Lrrk2 deficient and wild-type mice at different time-points after stimulation with insulin. N = 8 (for Thr308) and 6 (for Ser 473) independent experiments, normalized to Akt, mean ± SEM. (c) Western blot analysis of protein lysates from fibroblasts of 22 months old Lrrk2 deficient and wild-type rats at different time points after stimulation with insulin and (d) the corresponding quantification of P-Akt Thr308 (top) and P-Akt S473 (button) signal intensity. N = 10 independent experiments, normalized to Akt, mean ± SEM. (e) Western blot analysis of protein lysates from fibroblasts of 6 months old rats (wild-type and Lrrk2-deficient) after stimulation with insulin and corresponding quantification of P-Akt Thr308 (f) and S473 (g) signal intensity. N = 7 independent experiments, normalized to Akt, mean and SEM.

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