Figure 4
From: Altered proteome of high-density lipoproteins from paediatric acute lymphoblastic leukemia survivors
Expression of specific HDL-associated proteins. Proteins from isolated HDL were loaded onto stain-free gel, which has a linear dynamic detection range and allows for protein detection on both gels and membranes along with the use of total protein measurement. Following gel electrophoresis with stain-free gels and in the presence of MW proteins (A), imaging using the Chemidoc MP System to check protein separation quality, the blot was incubated with primary antibodies against (B) SAA1, (C) fibrinogen, and (D) integrin β-3. Thereafter, the blot was incubated with the secondary antibody, followed by imaging and data analysis by Image Lab Software. Results are means ± SEM and originated from at least three subjects per group. Given the variability observed among individuals, the whole gels were shown according to the trend of the calculated average. **p < 0,01; ***p < 0,001.
