Table 3 Primer sequences used for cDNA and gDNA amplification.
Primer name | Primer sequence | Expected amplicon size (bp) | Annealing t (°C) |
|---|---|---|---|
cDNA MLPH ex 6–9 F | TTTGAGGCTGACTCTGACGA | 486 | 60.0 |
cDNA MLPH ex 6–9 R | CCTCCTGAGGGTCTCCTCTT | ||
gDNA MLPH ex 7 F | CCTCCAGAAGAGCAGATGG | 335 | 58.0 |
gDNA MLPH ex 7 R | GAGCTATTGATGCTGGGACT | ||
cDNA MITF ex 1M-2 F | CTTCTCTATGCCCGTCAGTC | 241 | 57.5 |
cDNA MITF ex 1M-2 R | GGTTGGCATGTTTATTTGCT | ||
gDNA MITF ex 1 M F | CTTCTCTATGCCCGTCAGTC | 368 | 58.0 |
gDNA MITF ex 1 M R | GAACAGGAGCTGATGGAGAG | ||
сDNA B2M ex 1–2 F | TTCTCTGGACGTTGGTCTTC | 236 | 58.0 |
сDNA B2M ex 1–2 R | GAAACTCCAGTCCTTGCTGA |
