Figure 2
From: Developing a novel FRET assay, targeting the binding between Antizyme-AZIN
Measuring the Kd of the AZIN-antizyme (AZ) interaction by competition using fluorescent protein fusions. Clover-AZIN [1 µM] and AZ-mRuby2 [1 µM] were incubated to establish equilibrium. BFP2-AZ [48 nM-25 µM] was titrated to compete with AZ-mRuby2 for Clover-AZIN binding and the resulting FRET ratio was plotted.
