Figure 5
From: Developing a novel FRET assay, targeting the binding between Antizyme-AZIN
Mutational analysis of antizyme-AZIN binding. (A) The affinity of 39 single alanine mutants in AZIN for antizyme were measured using the Clover-AZIN: antizyme-mRubv2 FRET sensor. (B) Heatmap of AZIN structure. Red indicates positions where a substitution from the naturally occurring amino acid to an alanine increased affinity; blue indicates a decrease in affinity; purple indicates a marginal effect.
