Figure 2
From: An Effective Neutralizing Antibody Against Influenza Virus H1N1 from Human B Cells
32D6 selectively recognizes H1N1 clinical isolates. MDCK cells were infected with indicated clinical isolated H1N1 influenza virus strains. After 16 hours, infected cells were fixed by 4% paraformaldehyde then double stained by 32D6 (0.3 μg/mL) and FITC conjugated anti-influenza A NP antibody (0.67 μg/mL), followed by Alexa Fluor 647-conjugated mouse anti-human IgG. Nuclei were counterstained by DAPI (2 μg/mL). Signals were detected by high-content analysis system (10x). Blue = DAPI (nucleus); Green = anti-influenza A NP protein Ab-FITC; Red = 32D6 + mouse anti-human IgG-Fluor647. The genotype of each clinical isolate was listed in Table 1.
