Figure 3
From: An Effective Neutralizing Antibody Against Influenza Virus H1N1 from Human B Cells
Crystal structure of 32D6-Fab/HA1 complex. (a) 32D6-Fab/HA1 complex. The HA1 domain is represented as a green-colored ribbon, and the N-linked oligosaccharide at Asn104 is shown as green sticks. The heavy and light chains are shown in purple and yellow, respectively. The 32D6-Fab/HA1 complex structure is superimposed on the HA trimer (PDB 3LZG). The Fab on the top of the HA trimer is shown in purple and yellow surface for the heavy chain and the light chain, respectively. The trimeric structure of HA with the glycan (green sphere) is shown as a ribbon. Three different HA1 domains from the HA trimer are colored green, orange, and pink, respectively, and all HA2 domains are colored in gray. (b) HA1-32D6 interaction interface. The HA molecule is shown as a gray ribbon, and the epitope on HA is shown as stick models with green C atoms. The heavy-chain and light-chain CDRs are shown as purple and yellow ribbons, respectively. The residues involved in contact with HA from CDRs are indicated and are shown as sticks. Three water molecules that mediate the interaction are shown as cyan spheres. (c,d) Polar interactions and nonpolar interactions between HA1 and 32D6-Fab. The amino acid residues from each CDR and part of the HA sequence from H1N1 are shown. The interaction residues are colored in red, and each interaction is shown as a straight line. The inset shows the nonpolar interaction between the heavy chain CDR3 and the HA1. Three water molecules that mediate the polar interactions are also indicated.
