Figure 3
From: The DNA damage induced by the Cytosine Deaminase APOBEC3A Leads to the production of ROS
Both short and long A3A isoforms induce double strand breaks and production of ROS. (A) Short (A3A-S) and long A3A isoforms (A3A-L) were engineered by standard mutagenesis and stable HeLa cell lines were obtained as indicated in the legend to Fig. 2. The WB panels display typical expression profiles obtained and the migration of WT A3A is shown here solely for clarity’s sake (the same panel for WT A3A being presented in Fig. 2A). (B–D) After doxycycline induction (1 μg/mL), cells were analyzed by flow cytometry to measure either the levels of double strand breaks, as well as ROS. The histograms present typical results obtained out of 3 independent experiments. The graph presents averages and SEM of three independent experiments. Results obtained with WT-A3A are simply reported here for comparison. A Student t test was performed between the indicated conditions (two-tailed, unpaired; *p < 0,05).
