Figure 1 | Scientific Reports

Figure 1

From: The rsmS (ybaM) mutation causes bypass suppression of the RsmAB post-transcriptional virulence regulation system in enterobacterial phytopathogens

Figure 1

Mutation of rsmS affects biosynthesis of PCWDEs. (a) Genomic context of rsmS within Pba. Genes are indicated with arrows and names are written within. The numbers below indicate the number of bp between each gene. (b) The priC-rsmS promoter region. The −10 and −35 sites are indicated in bold. Putative binding sites for Crp, OxyR, and Lrp are indicated with a line under the site. A putative ArcA binding site is indicated in italics as it overlaps both the OxyR and −10 site. Putative Shine-Dalgarno sites are indicated as RBS and the translational start and stop sites are indicated in bold. (c) PCWDE plate assays showing production of protease (left), pectate lyase (middle) and cellulase (right) for the wild type, rsmB (NW155), rsmS (KA32) or rsmB rsmS (KA35) after incubation at 25 °C.

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