Figure 2

An rsmS mutation alters PCWDE and OHHL production. Levels of protease (a), cellulase (b), pectate lyase (c) and OHHL (d) in the culture supernatant determined at two-hour intervals throughout growth in PMB at 25 °C. Wild type (black), rsmS (KA32, blue), rsmB (NW155, green) and rsmB rsmS (KA35, red) cultures were assessed for growth (OD600 – dashed lines) and sterile supernatant samples taken every two hours. Solid lines indicate the enzyme activity or relative OHHL amounts determined from sterile supernatants. Values indicate the average +/− SD (n = 3).