Table 1 Strains, plasmids and bacteriophages used in this study.
Strain | Genotype | Reference |
|---|---|---|
Pectobacterium atrosepticum | ||
SCRI 1043 | Wild type | |
NW155 | rsmB::aph KnR | |
MC3 | rsmB::aph priC::TnDS1028 KnR CmR | This study |
KA32 | rsmS::cat CmR | This study |
KA35 | rsmB::aph rsmS::cat KnR CmR | This study |
Pectobacterium carotovorum | ||
ATCC39048 | Wild type | American Type Culture Collection |
SBEB | rsmB::Tn-cat CmR | This work |
SBES | rsmS::Tn5 KnR | This work |
SBEBY | hexY::Tn5, rsmB::TnDS1028 CmR KnR | This work |
SBEBS | rsmB::Tn-cat, rsmS::Tn5 CmR KnR | This work |
Escherichia coli | ||
ESS | β-lactam antibiotic sensor strain | |
β2163 | F- RP4-2-Tc::Mu, dapA::(erm-pir) | |
JM109 + pSM401 | OHHL sensor strain | |
Chromobacterium violaceum | ||
CV026 | cviI::Tn5; Acyl homoserine lactone indicator strain | |
Bacteriophages | ||
φM1 | Pba generalized transducing phage | |
φKP | Pcc39048 generalized transducing phage | |
Plasmids | ||
pBAD30 | Vector containing the araBAD promoter ApR | |
pBAD30-priC | priC coding sequence from SCRI 1043 in pBAD30 ApR | This study |
pBAD30-rsmS | rsmS coding sequence from SCRI 1043 in pBAD30 ApR | This study |
pBluescript II SK+ | blaM, lacZ ApR | Stratagene |
pBluescript-ΔrsmS | Regions surrounding rsmS from SCRI 1043 in pBluescript | This study |
pBluescript-ΔrsmS-Cm | cat gene in BglII site of pBluescript-ΔrsmS | This study |
pACYC184 | Vector containing cat gene, CmR | NEB |
pKNG101 | Allelic exchange vector StR | |
pKNG101-ΔrsmS-Cm | rsmS::CmR knockout cassette in pKNG101 StR | This study |
pDS1028 | Transposon containing plasmid used for random mutagenesis | |
