Table 2 Oligonucleotides used in this study.
Name | Sequence (5′-3′) | Details |
|---|---|---|
oKA17 | GGGGAATTCCGAGAAAGAAGAGGAAATG | Upstream oligo pBAD30-rsmS |
oKA28 | GGGAAGCTTTTAAGAGGAAGATTTAGCGTC | Downstream oligo pBAD30-rsmS |
oKA33 | GGGGATCCTGGCACTTGAACAACAAATTAACACGC | Creation of rsmS::CmR |
oKA34 | CCATTGCCAGTGCGGCGAGTGAGATCTCGATACGGGGTTCGA TTTGG | Creation of rsmS::CmR |
oKA35 | TCGAGATCTCACTCGCCGCACTGGCAATGG | Creation of rsmS::CmR |
oKA36 | GGGGGGCTCGAGACAGATACTGGATACTGCGTT | Creation of rsmS::CmR |
oKA42 | ACAGATCTGGCTATTTAACGACCC | Creation of rsmS::CmR |
oKA43 | TAAGATCTCGGAAGATCACTTCGCAG | Creation of rsmS::CmR |
oKA89 | GGGGAATTCCTACACAACGTCGGCCCGCTG | Upstream oligo pBAD30-priC |
oKA91 | GGAAGCTTCTACAGTCCTTGTTCCCGGCGTTCAATC | Downstream oligo pBAD30-priC |
oMAMV1 | GGAATTGATCCGGTGGATG | Sequencing oligo TnKRCPN1 |
oMAMV2 | GCATAAAGCTTGCTCAATCAATCAC | Sequencing oligo TnKRCPN1 |
oPF106 | GACCACACGTCGACTAGTGCNNNNNNNNNNAGAG | Random primed PCR oligo 1 |
oPF107 | GACCACACGTCGACTAGTGCNNNNNNNNNNACGCC | Random primed PCR oligo 2 |
oPF108 | GACCACACGTCGACTAGTGCNNNNNNNNNNGATAC | Random primed PCR oligo 3 |
oPF109 | GACCACACGTCGACTAGTGC | Random primed PCR adaptor oligo |
oREM7 | CTAGAGTCGACCTGCAGGC | Sequencing oligo TnDS1028 |
oREM8 | CACAGGAACACTTAACGGC | Sequencing oligo TnDS1028 |
