Figure 1
From: Skeletonema marinoi as a new genetic model for marine chain-forming diatoms
Genetic transformation of S. marinoi. (A) Schematic designs of the linear DNA constructs for the transformation of S. marinoi R05AC. Two different promoter/terminator regions were chosen to express the selectable marker gene (bleoR) conferring resistance to the antibiotic zeocin; fcpB, gene for the P. tricornutum fucoxanthin-chlorophyll protein; lsu4e, gene for the S. marinoi 80 S ribosomal large subunit protein 4e. Also shown are the two oligonucleotides (Bleo1 and –2) used to PCR amplify the bleoR gene. (B) Sensitivity of S. marinoi to the antibiotic zeocin relative to that of P. tricornutum. Growth was monitored daily by chlorophyll fluorescence for one week in the presence of different zeocin concentrations. Shown are averages and standard deviation of four replicate cultures. (C) Amplification of the bleoR gene with Bleo1 and Bleo2 primers in representative transformants using the fcpB:bleoR or lsu4e:bleoR constructs.
