Figure 3
From: Skeletonema marinoi as a new genetic model for marine chain-forming diatoms
Mapping the genomic insertion sites in several representative transformants using TAIL-PCR. TAIL-PCR was performed on genomic DNA extracted from the transformants SM1–2, SM10, SM12–13 and SM15 using one of the SAD1–4 degenerate primers in each reaction in combination with the construct-specific primers for either the promoter or terminator ends. The final PCR products were separated and visualized by gel electrophoresis as shown, with selected individual products then purified and sequenced.
