Figure 2

The effect of estrogen withdrawal and oscillatory fluid flow on MLO-Y4 cell morphology. (A) Immunocytochemistry images showing actin fibres and vinculin staining (N = 3, n ≥ 115 cells per group). Quantification of the images showing (B) cell area, (C) % focal adhesion area/cell area, (D) actin fluorescent intensity and (E) anisotropy of actin fibres. (Student’s t-test, *p < 0.05, **p < 0.01, ****p < 0.0001).