Figure 3
The effect of estrogen withdrawal and oscillatory fluid flow on αvβ3 quantity and organisation in MLO-Y4 cells. (A) Immunocytochemistry images showing αvβ3 and vinculin staining (N = 3, n ≥ 115 cells per group). (B) ELISA measurement of total αvβ3 quantity normalised to DNA content (N = 6). Quantification of the images showing (C) αvβ3 intensity per cell, and (D) αvβ3 intensity at focal adhesion (FA) sites (Student’s t-test, *p < 0.05, **p < 0.01, ****p < 0.0001).
