Figure 3 | Scientific Reports

Figure 3

From: A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads

Figure 3

Multiplex analysis. (a) Schematic representation of five different target oligonucleotides tested for multiplex analysis. Each target sequence is assigned to one microbead population with different fluorescent codes (MB n, n = 1–5). Red circle represents the reporter. (b) Normalised surface fluorescence signal on microbead for ssDNA (black squares) and DNA origami (red squares) according to target 1 concentration with each reporter at 2.5 nM. (c) Assay parameters (LoD, LoQ, EC50) determined from signal response plots for all targets all show similar values. As shown in Fig. 2c, clear distinction between ssDNA and DNA origami was shown with DNA origami showing significantly lower values for all three parameters (EC50 values shown on right axis).

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