Figure 1 | Scientific Reports

Figure 1

From: Molecular profiling of resident and infiltrating mononuclear phagocytes during rapid adult retinal degeneration using single-cell RNA sequencing

Figure 1

Immune cells respond to acute photoreceptor degeneration. (a) Immunohistochemical sections before and after onset of photoreceptor degeneration. After 48 hours of light exposure, CD11b+ cells that were round appeared in the vitreous and nerve fiber layer (NFL) near large caliber vessels while those that were ameboid were present in the subretinal space (SR) and photoreceptor layer (outer nuclear layer, ONL). Scale bars indicate 25 µm, INL = inner nuclear layer. (b) In vivo retinal imaging using scanning laser ophthalmoscopy. Cells expressing RFP driven by the CCR2 promoter (putative monocytes) appeared abruptly within 48 hours of light exposure (compare 0 to 48 hours exposure taken from the same mouse), while the number of GFP expressing resident microglia of changed little. Insets show corresponding reflectance images.

Back to article page