Figure 1
From: Moderate Nucleoporin 133 deficiency leads to glomerular damage in zebrafish
Expression of nup133 in the developing zebrafish detected by in situ hybridization (ISH). Whole mount ISH with nup133 antisense probe of embryos at: (a) sphere stage (4 hpf; embryo shown with animal pole to the top); (b) 24 hfp (lateral view); (c–f) 3 and 5 dpf (left panels: dorsal view; right panels: lateral view). Arrows point to tissues with enriched expression of nup133. Abbreviations: E: eyes; T: tectum; C: cerebellum; L: liver; I: intestine; N: neuromasts; SB: swim bladder. Scale bars, 200 µm. (g) Transverse section of a 5 dpf embryo at the level of the pectoral fins (as shown in the dotted line in e) confirms nup133 expression in the liver, and show in addition a diffuse staining in the proximal tubules (PT) and a faint signal in the glomerulus (G). Scale bar, 50 µm.
