Figure 7
From: Moderate Nucleoporin 133 deficiency leads to glomerular damage in zebrafish
nup133 knockdown induces moderate foot processes effacement. (a,d) Transmission electron micrographs showing the glomerular capillary wall of 5 dpf uninjected control (a) and nup133 MO injected larvae (d). (b,e) Two-fold magnification of the areas indicated in (a,d), and (c,f,h) representative images from distinct larvae. Images were pseudocolored to better highlight the glomerular filtration barrier components (podocyte foot processes in green, fenestrated endothelium in blue). Arrows point to irregular-shaped foot processes that are more frequently found in nup133 MO larvae. (g) Quantification of foot processes (FP) density along the basement membrane, presented as average number of FP/µm, was performed as described under materials and methods. The distinct labels reflect values measured on the 3 wt or morphant embryos. Statistical analyses were performed using Wilcoxon-Mann-Whitney Rank Sum Test. **P ≤ 0.01 (p = 0.002486). Image (h) corresponds to a basement membrane surrounding a large cyst (presented in Supplemental Fig. S6e). Note that statistical analyses performed without the latter nup133 morphant larva (values indicated by black squares on the graph) still revealed a significant difference between the control and the nup133 morphants (p = 0.01855). Abbreviations: BS: bowman space; C: capillary lumen; EC: endothelial cells; FP: foot processes. Scale bars 1 µm.
